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Article

The reduced levels of chi recognition exhibited by the RecBC1004D enzyme reflect its recombination defect in vivo.

The Journal of biological chemistry - 26 Jun 1998

Arnold D A, Bianco P R, Kowalczykowski S C

Abstract excerpt

Homologous recombination in Escherichia coli is initiated by the RecBCD enzyme and is stimulated by an 8-nucleotide element known as Chi (chi). We present a detailed biochemical characterization of a mutant RecBCD enzyme, designated RecBC1004D, that displays a reduced level of chi site recognition. Initially characterized genetically as unable to respond to the chi sequence, we provide evidence to indicate that...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Share to Recombination, Genetic

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Source

Identifiers

PMID: 9632715
DOI: 10.1074/jbc.273.26.16476

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

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Research trail→

Click a neighbor to travelStep 1 · 12 closest

The reduced levels of chi recognition exhibited by the RecBC1004D enzyme reflect its recombination defect in vivo.PMID 9632715Read ↗

A novel, 11 nucleotide variant of chi, chi*: one of a class of sequences defining the Escherichia coli recombination hotspot chi.Semantic proximity Read ↗

Biochemical characterization of a mutant recBCD enzyme, the recB2109CD enzyme, which lacks chi-specific, but not non-specific, nuclease activity.Semantic proximity Read ↗

Facilitated loading of RecA protein is essential to recombination by RecBCD enzyme.Semantic proximity Read ↗

A RecA mutant, RecA(730), suppresses the recombination deficiency of the RecBC(1004)D-chi* interaction in vitro and in vivo.Semantic proximity Read ↗

Reversible inactivation of the Escherichia coli RecBCD enzyme by the recombination hotspot chi in vitro: evidence for functional inactivation or loss of the RecD subunit.Semantic proximity Read ↗

Genetic dissection of the biochemical activities of RecBCD enzyme.Semantic proximity Read ↗

Nuclease activity is essential for RecBCD recombination in Escherichia coli.Semantic proximity Read ↗

Chi-star, a chi-related 11-mer sequence partially active in an E. coli recC1004 strain.Semantic proximity Read ↗

The mutant recBCD enzyme, recB2109CD enzyme, has helicase activity but does not promote efficient joint molecule formation in vitro.Semantic proximity Read ↗

A single mutation, RecB(D1080A,) eliminates RecA protein loading but not Chi recognition by RecBCD enzyme.Semantic proximity Read ↗

Chi hotspot activity in Escherichia coli without RecBCD exonuclease activity: implications for the mechanism of recombination.Semantic proximity Read ↗

Role of the Escherichia coli recombination hotspot, chi, in RecABCD-dependent homologous pairing.Semantic proximity Read ↗

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