Article
The reduced levels of chi recognition exhibited by the RecBC1004D enzyme reflect its recombination defect in vivo.
The Journal of biological chemistry - 26 Jun 1998
Arnold D A, Bianco P R, Kowalczykowski S C
Abstract excerpt
Homologous recombination in Escherichia coli is initiated by the RecBCD enzyme and is stimulated by an 8-nucleotide element known as Chi (chi). We present a detailed biochemical characterization of a mutant RecBCD enzyme, designated RecBC1004D, that displays a reduced level of chi site recognitio...
Topics
- Adenosine Triphosphatases
- DNA Helicases
- DNA, Bacterial
- DNA, Single-Stranded
- Escherichia coli
- Exodeoxyribonuclease V
- Exodeoxyribonucleases
- Exonucleases
- Mutation
- Nucleotides
- Recombination, Genetic
- Regulatory Sequences, Nucleic Acid
- Structure-Activity Relationship