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Article

Intersubunit signaling in RecBCD enzyme, a complex protein machine regulated by Chi hot spots.

Genes & development - 15 Dec 2007

Amundsen Susan K, Taylor Andrew F, Reddy Manjula, Smith Gerald R

Abstract excerpt

The Escherichia coli RecBCD helicase-nuclease, a paradigm of complex protein machines, initiates homologous genetic recombination and the repair of broken DNA. Starting at a duplex end, RecBCD unwinds DNA with its fast RecD helicase and slower RecB helicase on complementary strands. Upon encountering a Chi hot spot (5'-GCTGGTGG-3'), the enzyme produces a new 3' single-strand end and loads RecA protein onto it,...

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Identifiers

PMID: 18079176
DOI: 10.1101/gad.1605807
PMCID: PMC2113030

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Source receipt: pubmed, retrieved 7 Sept 2026.

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Intersubunit signaling in RecBCD enzyme, a complex protein machine regulated by Chi hot spots.PMID 18079176Read ↗

Chi hotspot control of RecBCD helicase-nuclease by long-range intramolecular signaling.Semantic proximity Read ↗

A single mutation, RecB(D1080A,) eliminates RecA protein loading but not Chi recognition by RecBCD enzyme.Semantic proximity Read ↗

Reversible inactivation of the Escherichia coli RecBCD enzyme by the recombination hotspot chi in vitro: evidence for functional inactivation or loss of the RecD subunit.Semantic proximity Read ↗

RecBCD enzyme switches lead motor subunits in response to chi recognition.Semantic proximity Read ↗

A domain of RecC required for assembly of the regulatory RecD subunit into the Escherichia coli RecBCD holoenzyme.Semantic proximity Read ↗

Translocation by the RecB motor is an absolute requirement for {chi}-recognition and RecA protein loading by RecBCD enzyme.Semantic proximity Read ↗

Small-molecule sensitization of RecBCD helicase-nuclease to a Chi hotspot-activated state.Semantic proximity Read ↗

A RecA mutant, RecA(730), suppresses the recombination deficiency of the RecBC(1004)D-chi* interaction in vitro and in vivo.Semantic proximity Read ↗

The recombination hot spot chi activates RecBCD recombination by converting Escherichia coli to a recD mutant phenocopy.Semantic proximity Read ↗

Nuclease activity is essential for RecBCD recombination in Escherichia coli.Semantic proximity Read ↗

Chi and the RecBC D enzyme of Escherichia coli.Semantic proximity Read ↗

A novel, 11 nucleotide variant of chi, chi*: one of a class of sequences defining the Escherichia coli recombination hotspot chi.Semantic proximity Read ↗

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