Article
Restriction landmark genomic scanning method and its various applications.
Electrophoresis - 1 Apr 1993
Hayashizaki Y, Hirotsune S, Okazaki Y, Hatada I, Shibata H, Kawai J, Hirose K, Watanabe S, Fushiki S, Wada S
Abstract excerpt
We have developed a new genome scanning method (restriction landmark genomic scanning (RLGS), based on the new concept of using restriction enzyme sites as landmarks. RLGS employs direct end labeling of the genomic DNA digested with a restriction enzyme and two-dimensional electrophoresis with hi...
Topics
- Animals
- Binding Sites
- DNA
- DNA Restriction Enzymes
- DNA, Neoplasm
- Electrophoresis, Gel, Two-Dimensional
- Gene Amplification
- Gene Deletion
- Methylation
- Mice
- Mutation