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Tagging Genes and Trapping Promoters inToxoplasma gondiiby Insertional Mutagenesis

1 Oct 1997

Abstract excerpt

Plasmid vectors that incorporate sequence elements from the dehydrofolate reductase-thymidylate synthase (DHFR-TS) locus of Toxoplasma gondii integrate into the parasite genome with remarkably high frequency (>1% of transfected parasites). These vectors may-but need not-include mutant DHFR-TS alleles that confer pyrimethamine resistance to transgenic parasites. Large genomic constructs integrate at the endogenous...

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PMID: 9405195
DOI: 10.1006/meth.1997.0504

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