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Combined gene deletion of dihydrofolate reductase-thymidylate synthase and pteridine reductase in Leishmania infantum.

PLoS neglected tropical diseases - 1 Apr 2021

Bhattacharya Arijit, Leprohon Philippe, Ouellette Marc

Abstract excerpt

Our understanding of folate metabolism in Leishmania has greatly benefited from studies of resistance to the inhibitor methotrexate (MTX). Folates are reduced in Leishmania by the bifunctional dihydrofolate reductase thymidylate synthase (DHFR-TS) and by pteridine reductase (PTR1). To further our understanding of folate metabolism in Leishmania, a Cos-seq genome-wide gain of function screen was performed against...

Topics

Animals
DNA, Protozoan
DNA, Recombinant
Drug Resistance
Folic Acid
Folic Acid Antagonists
Gene Deletion
Leishmania infantum
Methotrexate
Multienzyme Complexes
Phenotype

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Tetrahydrofolate Dehydrogenase

Thymidylate Synthase

Transfection

Source

Open in PubMed Open full text

Identifiers

PMID: 33905412
DOI: 10.1371/journal.pntd.0009377
PMCID: PMC8104401

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 31 Aug 2026.

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