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Article

Isolation of lambda repressor mutants with defects in cooperative operator binding.

Biochemistry - 7 Sept 1993

Beckett D, Burz D S, Ackers G K, Sauer R T

Abstract excerpt

A hybrid operator-promoter region was designed to aid in a screen for cooperativity mutants of the lambda repressor. In this system, lambda repressor mutants with defects in pairwise cooperative binding are unable to act as efficient transcriptional repressors. Four single amino acid substitutions in the C-terminal domain of the repressor were isolated. Studies of the DNA binding properties of the purified mutant...

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Identifiers

PMID: 8369279
DOI: 10.1021/bi00086a012

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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