Article
Genetic selection for mutations that impair the co-operative binding of lambda repressor.
Molecular microbiology - 1 Feb 1994
Benson N, Adams C, Youderian P
Abstract excerpt
Bacteriophage lambda repressor binds co-operatively to adjacent pairs of DNA target sites. A novel combination of positive genetic selections, involving two different operon fusions derived from P22 challenge phages, was used to isolate mutant lambda repressors that have lost the ability to bind...
Topics
- Amino Acid Sequence
- Bacteriophage lambda
- Base Sequence
- Binding Sites
- DNA Mutational Analysis
- DNA-Binding Proteins
- Molecular Sequence Data
- Mutation
- Oligodeoxyribonucleotides
- Protein Binding
- Repressor Proteins
- Selection, Genetic