Article
Rifampicin region revisited. New rifampicin-resistant and streptolydigin-resistant mutants in the beta subunit of Escherichia coli RNA polymerase.
The Journal of biological chemistry - 15 Jul 1993
Severinov K, Soushko M, Goldfarb A, Nikiforov V
Abstract excerpt
Mutations to rifampicin resistance (RifR) in Escherichia coli alter the beta subunit of RNA polymerase (RNAP). A series of new point RifR mutations was isolated with the aid of random polymerase chain reaction-mediated mutagenesis followed by selection on rifampicin (Rif). All of the new RifR mut...
Topics
- Amino Acid Sequence
- Aminoglycosides
- Anti-Bacterial Agents
- Base Sequence
- DNA, Single-Stranded
- DNA-Directed RNA Polymerases
- Drug Resistance, Microbial
- Escherichia coli
- Molecular Sequence Data
- Mutagenesis, Insertional
- Mutagenesis, Site-Directed
- Mutation
- Rifampin
- Sequence Alignment
- Sequence Deletion
- Transcription, Genetic