Article
Four contiguous amino acids define the target for streptolydigin resistance in the beta subunit of Escherichia coli RNA polymerase.
The Journal of biological chemistry - 5 Dec 1993
Heisler L M, Suzuki H, Landick R, Gross C A
Abstract excerpt
Streptolydigin (stl), a bacteriostatic inhibitor of transcription elongation, interacts with the beta subunit of Escherichia coli RNA polymerase. We have defined the target for stl resistance using chemical mutagenesis and mutagenic polymerase chain reaction. Mutations resulting in stl resistance...
Topics
- Amino Acid Sequence
- Aminoglycosides
- Anti-Bacterial Agents
- Base Sequence
- DNA, Bacterial
- DNA-Directed RNA Polymerases
- Drug Resistance, Microbial
- Escherichia coli
- Genes, Bacterial
- Genotype
- Kinetics
- Macromolecular Substances
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Polymerase Chain Reaction
- Restriction Mapping