Article
Determination of allele frequencies at loci with length polymorphism by quantitative analysis of DNA amplified from pooled samples.
PCR methods and applications - 1 May 1993
Pacek P, Sajantila A, Syvänen A C
Abstract excerpt
We present a new method that allows rapid determination of allele frequencies at loci exhibiting length polymorphism. In this method a fluorescence-labeled PCR primer is used to amplify the polymorphic region from pooled DNA samples originating from a large number of individuals. The fluorescent PCR products are separated by gel electrophoresis on an automatic DNA sequencer and the relative amount of the PCR...
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