Article
Estimation of SNP allele frequencies by SSCP analysis of pooled DNA.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2009
Tahira Tomoko, Kukita Yoji, Higasa Koichiro, Okazaki Yuko, Yoshinaga Aki, Hayashi Kenshi
Abstract excerpt
The single strand conformation polymorphism (SSCP) method is a sensitive technique used to detect subtle sequence differences in PCR-amplified DNA fragments as separated peaks in electrophoretic analysis. In this chapter, we focus on SSCP analysis for quantifying polymorphic alleles rather than scanning for mutations. Short fragments carrying single nucleotide polymorphisms are amplified from individual and...
Read the complete abstract on PubMedTopics
- DNA
- Electrophoresis, Capillary
- Fluorescent Dyes
- Gene Frequency
- Genetic Markers
- Humans
- Nucleotides