Article
Accumulation of p53 protein as an indicator for p53 gene mutation in breast cancer. Occurrence of false-positives and false-negatives.
Diagnostic molecular pathology : the American journal of surgical pathology, part B - 1 Mar 1993
Lohmann D, Ruhri C, Schmitt M, Graeff H, Höfler H
Abstract excerpt
Accumulation of p53 protein resulting in levels detectable by immunohistochemistry (IHC) has been proposed as an indicator of mutation of the p53 gene. We have investigated a panel of 23 fresh-frozen breast cancers by IHC (PAb 1801), Southern and Northern blot analysis, and direct sequencing of t...
Topics
- Base Sequence
- Blotting, Northern
- Blotting, Southern
- Breast Neoplasms
- DNA Mutational Analysis
- Genes, p53
- Humans
- Immunohistochemistry
- Molecular Sequence Data
- Mutation
- Sensitivity and Specificity