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Article

Random mutagenesis of the thymidine kinase gene of varicella-zoster virus.

Journal of virology - 1 Apr 1992

Suzutani T, Lacey S F, Powell K L, Purifoy D J, Honess R W

Abstract excerpt

To understand the relationship between the primary structure and function of varicella-zoster virus thymidine kinase (VZV TK; EC 2.7.1.21), we established rapid screening and phenotypic selection of mutant VZV TK genes in TK-deficient Escherichia coli C600 by using a constitutive pKK223-3 expression plasmid. In this screening system, mutant TK genes generated by random mutagenesis were identified by the...

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Identifiers

PMID: 1312622
DOI: 10.1128/JVI.66.4.2118-2124.1992
PMCID: PMC289003

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