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Article

Random mutagenesis of the thymidine kinase gene of varicella-zoster virus.

Journal of virology - 1 Apr 1992

Suzutani T, Lacey S F, Powell K L, Purifoy D J, Honess R W

Abstract excerpt

To understand the relationship between the primary structure and function of varicella-zoster virus thymidine kinase (VZV TK; EC 2.7.1.21), we established rapid screening and phenotypic selection of mutant VZV TK genes in TK-deficient Escherichia coli C600 by using a constitutive pKK223-3 expression plasmid. In this screening system, mutant TK genes generated by random mutagenesis were identified by the...

Topics

Amino Acid Sequence
Base Sequence
Binding Sites
Cloning, Molecular
DNA Mutational Analysis
DNA, Viral
Drug Resistance, Microbial
Herpesvirus 3, Human
Molecular Sequence Data
Mutagenesis
Phenotype

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Phosphorylation

Thymidine Kinase

Source

Open in PubMed Open full text

Identifiers

PMID: 1312622
DOI: 10.1128/JVI.66.4.2118-2124.1992
PMCID: PMC289003

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1312622-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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