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Article

RNase P ribozymes selected in vitro to cleave a viral mRNA effectively inhibit its expression in cell culture.

The Journal of biological chemistry - 7 Apr 2000

Kilani A F, Trang P, Jo S, Hsu A, Kim J, Nepomuceno E, Liou K, Liu F

Abstract excerpt

An in vitro selection procedure was used to select RNase P ribozyme variants that efficiently cleaved the sequence of the mRNA encoding thymidine kinase of herpes simplex virus 1. Of the 45 selected variants sequenced, 25 ribozymes carried a common mutation at nucleotides 224 and 225 of RNase P catalytic RNA from Escherichia coli (G(224)G(225) --> AA). These selected ribozymes exhibited at least 10 times higher...

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Source

Identifiers

PMID: 10744757
DOI: 10.1074/jbc.275.14.10611

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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