Article
Monoclonal antibodies that recognize antigens in human osteosarcoma cells and normal fetal osteoblasts.
Bone and mineral - 1 Sept 1993
Yamaguchi A, Kahn A J
Abstract excerpt
Four monoclonal antibodies (HOB-1-4) that react against human osteosarcoma cells (Saos-2) and human fetal osteoblasts in situ were developed by immunizing mice with Saos-2 cells. HOB-1 (IgG1, k) and HOB-2 (IgM, k) stain cytoplasmic antigens in Saos-2 cells, human authentic osteoblasts and occasio...
Topics
- Alkaline Phosphatase
- Animals
- Antibodies, Monoclonal
- Antigen-Antibody Reactions
- Blotting, Western
- Electrophoresis, Polyacrylamide Gel
- Embryonic and Fetal Development
- Frozen Sections
- Humans
- Immunohistochemistry
- Mice
- Mice, Inbred BALB C
- Osteoblasts
- Osteosarcoma
- Phenotype
- Precipitin Tests
- Tumor Cells, Cultured