Article
A serum substitute promotes osteoblast-like phenotypic expression in cultured cells from chick calvariae.
Bone and mineral - 1 Oct 1991
Ramp W K, Dillaman R M, Lenz L G, Gay D M, Roer R D, Ballard T A
Abstract excerpt
The effects of medium supplements were tested on embryonic chick calvarial cells in culture. Isolates were divided among four treatment groups: Nu-Serum, chicken serum, fetal bovine serum, or calf serum. Expression of the osteoblastic phenotype was assessed by cell morphology, DNA content, [3H]thymidine incorporation, lactate production, cellular and medium alkaline phosphatase activities, and collagen synthesis....
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