Article
NIH 3T3 cells stably transfected with the gene encoding phosphatidylcholine-hydrolyzing phospholipase C from Bacillus cereus acquire a transformed phenotype.
Molecular and cellular biology - 1 Jan 1994
Johansen T, Bjørkøy G, Overvatn A, Diaz-Meco M T, Traavik T, Moscat J
Abstract excerpt
In order to determine whether chronic elevation of intracellular diacylglycerol levels generated by hydrolysis of phosphatidylcholine (PC) by PC-hydrolyzing phospholipase C (PC-PLC) is oncogenic, we generated stable transfectants of NIH 3T3 cells expressing the gene encoding PC-PLC from Bacillus...
Topics
- 3T3 Cells
- Animals
- Bacillus cereus
- Base Sequence
- Cell Cycle
- DNA
- DNA, Antisense
- DNA, Bacterial
- Diglycerides
- Genetic Vectors
- Hydrolysis
- Mice
- Molecular Sequence Data
- Phenotype
- Phosphatidylcholines
- Transfection
- Type C Phospholipases