Article
Isolation and characterization of a dihydrofolate reductase gene mutation in methotrexate-resistant Drosophila cells.
Gene expression - 1 Jan 1996
Hao H, Tyshenko M G, Walker V K
Abstract excerpt
Stepwise increases in methotrexate (MTX) concentration over a 4-year period led to the selection of a highly drug-resistant (2 x 10(-4) M MTX) Drosophila cell line. Uptake experiments with [3H]MTX showed a slightly lower level of intracellular MTX in the resistant S3Mtx cells than in the susceptible S3 parental cell line. Southern blot analysis demonstrated that the gene for the MTX target, dihydrofolate...
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