Reconstitution of mammalian excision repair activity with mutant cell-free extracts and XPAC and ERCC1 proteins expressed in Escherichia coli.

doi:10.1093/nar/21.22.5110

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Back to search

Article

Reconstitution of mammalian excision repair activity with mutant cell-free extracts and XPAC and ERCC1 proteins expressed in Escherichia coli.

Nucleic acids research - 11 Nov 1993

Park C H, Sancar A

Abstract excerpt

Nucleotide excision repair in humans involves the coordinated actions of 8-10 proteins. To understand the roles of each of these proteins in excision it is necessary to develop an in vitro excision repair system reconstituted entirely from purified proteins. Towards this goal we have expressed in...

Topics

ATP-Binding Cassette Transporters
Carrier Proteins
Cell-Free System
Cloning, Molecular
DNA Damage
DNA Repair
DNA-Binding Proteins
Endonucleases
Escherichia coli
Escherichia coli Proteins
Genetic Complementation Test

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Humans

Maltose

Maltose-Binding Proteins

Monosaccharide Transport Proteins

Mutation

Proteins

Recombinant Proteins

Source

Open in PubMed Open full text

Identifiers

PMID: 8255764
DOI: 10.1093/nar/21.22.5110
PMCID: PMC310624

Genes

ERCC1
MBP

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-8255764-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Reconstitution of mammalian excision repair activity with mutant cell-free extracts and XPAC and ERCC1 proteins expressed in Escherichia coli.PMID 8255764Read ↗

Activity of individual ERCC1 and XPF subunits in DNA nucleotide excision repair.Semantic proximity Read ↗

Reconstitution of 5′-Directed Human Mismatch Repair in a Purified SystemSemantic proximity Read ↗

Requirement for ERCC-1 and ERCC-3 gene products in DNA excision repair in vitro. Complementation using rodent and human cell extracts.Semantic proximity Read ↗

Evidence for a repair enzyme complex involving ERCC1 and complementing activities of ERCC4, ERCC11 and xeroderma pigmentosum group F.Semantic proximity Read ↗

Affinity purification and partial characterization of a yeast multiprotein complex for nucleotide excision repair using histidine-tagged Rad14 protein.Semantic proximity Read ↗

The human DNA repair gene, ERCC2 (XPD), corrects ultraviolet hypersensitivity and ultraviolet hypermutability of a shuttle vector replicated in xeroderma pigmentosum group D cells.Semantic proximity Read ↗

Haemophilus influenzae UvrA: overexpression, purification, and in cell complementation.Semantic proximity Read ↗

Structural and mutational analysis of the xeroderma pigmentosum group D (XPD) gene.Semantic proximity Read ↗

Functional complementation of xeroderma pigmentosum complementation group E by replication protein A in an in vitro system.Semantic proximity Read ↗

Reconstitution of concerted DNA integration with purified components.Semantic proximity Read ↗

Two separable protein species which both restore uvrABC endonuclease activity in extracts from uvrC mutated cells.Semantic proximity Read ↗

Expression and assembly of a functional E1 component (alpha 2 beta 2) of mammalian branched-chain alpha-ketoacid dehydrogenase complex in Escherichia coli.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.