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Article

Expression and assembly of a functional E1 component (alpha 2 beta 2) of mammalian branched-chain alpha-ketoacid dehydrogenase complex in Escherichia coli.

The Journal of biological chemistry - 15 Aug 1992

Davie J R, Wynn R M, Cox R P, Chuang D T

Abstract excerpt

We have expressed an active recombinant E1 decarboxylase component of the mammalian branched-chain alpha-ketoacid dehydrogenase complex in Escherichia coli by subcloning mature E1 alpha and E1 beta subunit cDNA sequences into a bacterial expression vector. To permit affinity purification under native conditions, the mature E1 alpha subunit was fused with the affinity ligand E. coli maltose-binding protein (MBP)...

Topics

3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide)
ATP-Binding Cassette Transporters
Amino Acid Sequence
Animals
Base Sequence
Carrier Proteins
Cattle
Chromatography, Gel
Cloning, Molecular
DNA

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Escherichia coli

Escherichia coli Proteins

Factor Xa

Genetic Vectors

Humans

Ketone Oxidoreductases

Macromolecular Substances

Maltose

Source

Identifiers

PMID: 1644840

Genes

MBP

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1644840-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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