Article
Affinity purification and partial characterization of a yeast multiprotein complex for nucleotide excision repair using histidine-tagged Rad14 protein.
The Journal of biological chemistry - 18 Dec 1998
Rodriguez K, Talamantez J, Huang W, Reed S H, Wang Z, Chen L, Feaver W J, Friedberg E C, Tomkinson A E
Abstract excerpt
The nucleotide excision repair (NER) pathway of eukaryotes involves approximately 30 polypeptides. Reconstitution of this pathway with purified components is consistent with the sequential assembly of NER proteins at the DNA lesion. However, recent studies have suggested that NER proteins may be...
Topics
- Chromatography, Affinity
- DNA Repair
- DNA Repair Enzymes
- DNA, Fungal
- DNA, Superhelical
- Dose-Response Relationship, Radiation
- Fungal Proteins
- Histidine
- Phenotype
- Recombinant Proteins
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Ultraviolet Rays