Article
Dimerization specificity of myogenic helix-loop-helix DNA-binding factors directed by nonconserved hydrophilic residues.
Genes & development - 1 Dec 1993
Shirakata M, Friedman F K, Wei Q, Paterson B M
Abstract excerpt
The myogenic regulatory factor MyoD dimerizes with other positive and negative regulatory factors through a conserved region called the helix-loop-helix (HLH) domain. Using a non-DNA-binding MyoD mutant with a normal HLH domain as a dimerization competitor in gel mobility shift assays in conjunct...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Biopolymers
- Cells, Cultured
- DNA-Binding Proteins
- Helix-Loop-Helix Motifs
- Mice
- Molecular Sequence Data
- Mutation
- MyoD Protein
- Phenylalanine
- Recombinant Fusion Proteins
- TCF Transcription Factors
- Transcription Factor 7-Like 1 Protein
- Transcription Factors
- Transcriptional Activation