Article
HPA-1 typing by PCR amplification with sequence-specific primers (PCR-SSP): a rapid and simple technique.
British journal of haematology - 1 Sept 1993
Metcalfe P, Waters A H
Abstract excerpt
A DNA-based method was developed to genotype donors for the human platelet antigens HPA-1a and -1b. Sequence-specific primers (SSP) were used in the polymerase chain reaction (PCR) which allowed the HPA-1a/1a, -1b/1b and -1a/1b genotypes to be determined by PCR alone, no second analytical stage was required. 10 donors were tested by PCR-SSP and the results were concordant with serological phenotyping and...
Topics
- Antigens, Human Platelet
- Base Sequence
- DNA
- Gene Amplification
- Genotype
- Humans
- Molecular Sequence Data
- Polymerase Chain Reaction