Article
Human platelet antigens: typing by PCR using sequence-specific primers and their distribution in blood donors resident in Wales.
European journal of immunogenetics : official journal of the British Society for Histocompatibility and Immunogenetics - 1 Dec 1999
Sellers J, Thompson J, Guttridge M G, Darke C
Abstract excerpt
We developed and validated HPA-1 to HPA-6 typing by PCR-SSP using a combination of established, modified and newly designed sequence-specific primers. We confirmed that the PCR primer mixtures functioned under the same PCR conditions as our standard HLA-A, -B, -C, -DR, -DQ PCR-SSP typing system. This allows concurrent testing for both HPA and HLA specificities and is therefore the system of choice for both...
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