Rapid Typing for Human Platelet Antigen Systems ‐1, ‐2, ‐3 and ‐5 by PCR Amplification with Sequence‐Specific Primers

Abstract excerpt

Typing for human platelet antigens (HPA) is useful in a variety of clinical situations. We developed a method for genotyping for HPA-1, -2, -3 and -5 by means of the PCR amplification with sequence-specific primers (PCR-SSP) technique. Primer sets were designed to allow PCR amplification for all systems using the same assay conditions. Specificity and sensitivity of the method were assessed in a blind quality...