Genotyping of the human platelet antigen-1 by ELISA detection of allele-specific amplicons.

doi:10.1046/j.1423-0410.1997.7330185.x

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Article

Genotyping of the human platelet antigen-1 by ELISA detection of allele-specific amplicons.

Vox sanguinis - 1 Jan 1997

Müller T H, Döscher A, Schunter F

Abstract excerpt

BACKGROUND AND OBJECTIVES: The purpose of the study was to establish a valid and efficient method for genotyping of the human platelet alloantigen-1 (HPA-1) in large sample numbers. MATERIALS AND METHODS: Digoxigenin- and fluorescein-labelled allele-specific primers and a biotinylated common prim...

Topics

Alleles
Antigens, Human Platelet
Enzyme-Linked Immunosorbent Assay
Genotype
Humans
Polymerase Chain Reaction
Reproducibility of Results
Sensitivity and Specificity

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