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Article

Human platelet antigen-5 (Br) genotyping by ASPA: allele-specific primer amplification (PCR-SSP)

British journal of haematology - 1 Nov 1994

Simsek S, Bleeker P M, Heeremans J, von dem Borne A E

Abstract excerpt

We have developed a PCR assay named ASPA (allele-specific primer amplification) to determine the HPA-5a and -5b genotypes. It consists of two PCR-reactions. One primer of each primer set has a 3'-end nucleotide which is specific for A or G at position 505 of the HPA-5b or -5a allele respectively. The HPA-5 genotypes determined in this way were strictly concordant with the genotypes established by the PCR-ASRA and...

Topics

Alleles
Antigens, Human Platelet
Base Sequence
Exons
Genotype
Humans
Molecular Sequence Data
Polymerase Chain Reaction
Thrombocytopenia

Source

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 7819088
DOI: 10.1111/j.1365-2141.1994.tb05096.x

Genes

ASPA

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7819088-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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