Article
Screens for extragenic mutations that fail to complement act1 alleles identify genes that are important for actin function in Saccharomyces cerevisiae.
Genetics - 1 Oct 1993
Welch M D, Vinh D B, Okamura H H, Drubin D G
Abstract excerpt
Null mutations in SAC6 and ABP1, genes that encode actin-binding proteins, failed to complement the temperature-sensitive phenotype caused by a mutation in the ACT1 gene. To identify novel genes whose protein products interact with actin, mutations that fail to complement act1-1 or act1-4, two temperature-sensitive alleles of ACT1, were isolated. A total of 14 extragenic noncomplementing mutations and 12 new...
Topics
- Actins
- Alleles
- Genes, Fungal
- Genetic Complementation Test
- Microfilament Proteins
- Mutagenesis
- Phenotype
- Saccharomyces cerevisiae
- Temperature
- Transformation, Genetic