Article
Suppressor analysis of fimbrin (Sac6p) overexpression in yeast.
Genetics - 1 Apr 1999
Sandrock T M, Brower S M, Toenjes K A, Adams A E
Abstract excerpt
Yeast fimbrin (Sac6p) is an actin filament-bundling protein that is lethal when overexpressed. To identify the basis for this lethality, we sought mutations that can suppress it. A total of 1326 suppressor mutations were isolated and analyzed. As the vast majority of mutations were expected to simply decrease the expression of Sac6p to tolerable levels, a rapid screen was devised to eliminate these mutations. A...
Topics
- Actins
- Alleles
- Base Sequence
- Binding Sites
- DNA Primers
- Fungal Proteins
- Gene Expression
- Genome, Fungal
- Membrane Glycoproteins
- Microfilament Proteins
- Models, Molecular
- Mutation
- Phenotype
- Protein Conformation
- Saccharomyces cerevisiae
- Suppression, Genetic