Article
Structural and functional dissection of the Abp1 ADFH actin-binding domain reveals versatile in vivo adapter functions.
Molecular biology of the cell - 1 Jul 2005
Quintero-Monzon Omar, Rodal Avital A, Strokopytov Boris, Almo Steven C, Goode Bruce L
Abstract excerpt
Abp1 is a multidomain protein that regulates the Arp2/3 complex and links proteins involved in endocytosis to the actin cytoskeleton. All of the proposed cellular functions of Abp1 involve actin filament binding, yet the actin binding site(s) on Abp1 have not been identified, nor has the importance of actin binding for Abp1 localization and function in vivo been tested. Here, we report the crystal structure of...
Topics
- Actins
- Alleles
- Amino Acid Sequence
- Binding Sites
- Crystallography, X-Ray
- Endocytosis
- Green Fluorescent Proteins
- Immunoblotting
- Kinetics
- Ligands
- Microfilament Proteins
- Microscopy, Fluorescence
- Models, Molecular