A rapid screening method to detect nonsense and frameshift mutations: identification of disease-causing APC alleles.

Article

A rapid screening method to detect nonsense and frameshift mutations: identification of disease-causing APC alleles.

Cancer research - 1 Dec 1993

Varesco L, Groden J, Spirio L, Robertson M, Weiss R, Gismondi V, Ferrara G B, White R

Abstract excerpt

A functional screen for nonsense and frameshift mutations has been devised that allows genes of interest to be scanned in segments. This assay is based on the cloning of these segments in-frame with a colorimetric marker gene (lacZ) followed by screening for the level of functional activity from...

Topics

Alleles
Base Sequence
DNA Mutational Analysis
Frameshift Mutation
Genes, APC
Humans
Molecular Sequence Data

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