Article
Negative growth selection against rodent fibroblasts targeted for genetic inhibition of farnesyl transferase.
Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research - 1 Sept 1993
Prendergast G C, Davide J P, Kral A, Diehl R, Gibbs J B, Omer C A, Kohl N E
Abstract excerpt
The Ras oncoprotein must be modified by farnesyl transferase (FTase) for biological activity. Therefore, inhibition of FTase may offer a means to block ras induced cell transformation. To address this hypothesis, we have introduced antisense and dominant inhibitory FTase expression plasmids into a panel of normal, mutant ras-, and mos- transformed rodent fibroblasts in an effort to genetically suppress FTase...
Topics
- Alkyl and Aryl Transferases
- Animals
- Cell Division
- Cell Line, Transformed
- Farnesyltranstransferase
- Fibroblasts
- Genetic Vectors
- Humans
- Mutation
- RNA, Antisense