Article
Physical analysis of spontaneous and mutagen-induced mutants of Escherichia coli K-12 expressing DNA exonuclease VIII activity.
Genetics - 1 Jun 1990
Mahajan S K, Chu C C, Willis D K, Templin A, Clark A J
Abstract excerpt
We have mapped the extents of two deletion sbcA mutations which result in production of DNA exonuclease VIII (ExoVIII). One mutation, sbcA8, deletes about 140 kb of DNA which includes most of the Rac prophage and the trg gene. Western blot analysis shows that the protein produced is larger than wild type ExoVIII. The nucleotide sequence shows that a translational gene fusion has occurred. The N-terminal 294...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Blotting, Western
- Cloning, Molecular
- Escherichia coli
- Escherichia coli Proteins
- Exodeoxyribonucleases
- Gene Expression
- Genes, Bacterial
- Molecular Sequence Data