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Article

Stable, selectable, integrative DNA transformation in Physarum.

Gene - 15 Oct 1993

Burland T G, Bailey J, Pallotta D, Dove W F

Abstract excerpt

The Physarum polycephalum actin promoter, PardC, can drive transient expression of heterologous genes in Physarum amoebae. The hph gene, encoding hygromycin (Hy) phosphotransferase, can confer resistance to Hy on a broad spectrum of organisms. When PardC is translationally fused to hph and transformed into yeasts on high-copy-number vectors, the yeasts become Hy resistant (HyR), showing that PardC-hph is a...

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PMID: 8224865
DOI: 10.1016/0378-1119(93)90197-b

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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