Article
Stable, selectable, integrative DNA transformation in Physarum.
Gene - 15 Oct 1993
Burland T G, Bailey J, Pallotta D, Dove W F
Abstract excerpt
The Physarum polycephalum actin promoter, PardC, can drive transient expression of heterologous genes in Physarum amoebae. The hph gene, encoding hygromycin (Hy) phosphotransferase, can confer resistance to Hy on a broad spectrum of organisms. When PardC is translationally fused to hph and transf...
Topics
- Actins
- Animals
- Blotting, Southern
- Cinnamates
- Hygromycin B
- Phenotype
- Phosphotransferases (Alcohol Group Acceptor)
- Physarum
- Plasmids
- Promoter Regions, Genetic
- Restriction Mapping
- Transformation, Genetic