Cloning and manipulation of the Schizosaccharomyces pombe his7+ gene as a new selectable marker for molecular genetic studies.

doi:10.1007/BF00351711

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Article

Cloning and manipulation of the Schizosaccharomyces pombe his7+ gene as a new selectable marker for molecular genetic studies.

Current genetics - 1 Dec 1993

Apolinario E, Nocero M, Jin M, Hoffman C S

Abstract excerpt

We have cloned the his7+ gene of the fission yeast Schizosaccharomyces pombe by complementation of the recessive mutant allele his7-366. The his7+ gene is able to complement a mutation of the Escherichia coli hisI gene, suggesting that his7+ encodes a phosphoribosyl-AMP cyclohydrase. Subcloning e...

Topics

Cloning, Molecular
DNA, Recombinant
Escherichia coli
Genes, Fungal
Genetic Complementation Test
Genetic Markers
Genotype
Plasmids
Restriction Mapping
Schizosaccharomyces

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