Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter.

doi:10.1128/jb.177.14.4121-4130.1995

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Article

Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter.

Journal of bacteriology - 1 Jul 1995

Guzman L M, Belin D, Carson M J, Beckwith J

Abstract excerpt

We have constructed a series of plasmid vectors (pBAD vectors) containing the PBAD promoter of the araBAD (arabinose) operon and the gene encoding the positive and negative regulator of this promoter, araC. Using the phoA gene and phoA fusions to monitor expression in these vectors, we show that...

Topics

Alkaline Phosphatase
AraC Transcription Factor
Arabinose
Bacterial Proteins
Base Sequence
Cloning, Molecular
Enzyme Induction
Enzyme Repression
Escherichia coli
Escherichia coli Proteins
Gene Expression Regulation, Enzymologic

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Genes, Lethal

Genetic Vectors

Molecular Sequence Data

Mutation

Operon

Phosphotransferases (Alcohol Group Acceptor)

Promoter Regions, Genetic

Source

Open in PubMed Open full text

Identifiers

PMID: 7608087
DOI: 10.1128/jb.177.14.4121-4130.1995
PMCID: PMC177145

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-7608087-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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