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Article

Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter.

Journal of bacteriology - 1 Jul 1995

Guzman L M, Belin D, Carson M J, Beckwith J

Abstract excerpt

We have constructed a series of plasmid vectors (pBAD vectors) containing the PBAD promoter of the araBAD (arabinose) operon and the gene encoding the positive and negative regulator of this promoter, araC. Using the phoA gene and phoA fusions to monitor expression in these vectors, we show that the ratio of induction/repression can be 1,200-fold, compared with 50-fold for PTAC-based vectors. phoA expression can...

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Identifiers

PMID: 7608087
DOI: 10.1128/jb.177.14.4121-4130.1995
PMCID: PMC177145

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Source receipt: pubmed, retrieved 7 Sept 2026.

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