Integrative transformation of the ascomycete Podospora anserina: identification of the mating-type locus on chromosome VII of electrophoretically separated chromosomes.

doi:10.1007/BF00180633

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Article

Integrative transformation of the ascomycete Podospora anserina: identification of the mating-type locus on chromosome VII of electrophoretically separated chromosomes.

Applied microbiology and biotechnology - 1 Apr 1991

Osiewacz H D, Skaletz A, Esser K

Abstract excerpt

Protoplasts of wild-type strain s and a long-lived extrachromosomal mutant (AL2) of the ascomycete Podospora anserina were transformed using a plasmid (pAN7-1) which contains the hygromycin B phosphotransferase gene (hph) of Escherichia coli under the control of Aspergillus nidulans regulatory se...

Topics

Ascomycota
Benomyl
Cloning, Molecular
DNA, Fungal
Genes, Fungal
Genes, Mating Type, Fungal
Genetic Linkage
Hygromycin B
Mitosis
Mutation
Plasmids
Transformation, Genetic

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