Article
Transformation of Sordaria macrospora to hygromycin B resistance: characterization of transformants by electrophoretic karyotyping and tetrad analysis.
Current genetics - 1 Dec 1995
Walz M, Kück U
Abstract excerpt
The ascomycete Sordaria macrospora was transformed using different plasmid molecules containing the bacterial hygromycin B resistance gene (hph) under the control of different expression signals. The highest transformation frequency was obtained with vector pMW1. On this plasmid molecule, express...
Topics
- Ascomycota
- Chromosome Mapping
- Crosses, Genetic
- Drug Resistance, Microbial
- Electrophoresis, Gel, Pulsed-Field
- Escherichia coli
- Gene Expression Regulation
- Genetic Markers
- Hygromycin B
- Karyotyping
- Meiosis
- Oxidoreductases
- Phenotype
- Promoter Regions, Genetic
- Recombinant Proteins
- Spores, Fungal
- Transformation, Genetic