Article
A new versatile system for rapid control of gene expression in the fission yeast Schizosaccharomyces pombe.
Yeast (Chichester, England) - 1 Oct 2012
Zilio Nicola, Wehrkamp-Richter Sophie, Boddy Michael Nicholas
Abstract excerpt
The ability to regulate the expression of a gene greatly aids the process of uncovering its functions. The fission yeast Schizosaccharomyces pombe has so far lacked a system for rapidly controlling the expression of chromosomal genes, hindering its full potential as a model organism. Although the widely used nmt1 promoter displays a wide dynamic range of activity, it takes > 14-15 h to derepress. The urg1...
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