Article
A cosmid-based system for constructing mutants of herpes simplex virus type 1.
Virology - 1 Nov 1993
Cunningham C, Davison A J
Abstract excerpt
Cosmids containing large fragments of herpes simplex virus type 1 DNA were prepared using a vector that allows intact inserts to be excised using the restriction endonuclease PacI. Two independent sets (A and B) of five cosmids were identified whose inserts overlap and represent the entire viral...
Topics
- Animals
- Cell Line
- Cloning, Molecular
- Cosmids
- Cricetinae
- DNA Replication
- DNA, Viral
- Gene Deletion
- Gene Library
- Genes, Viral
- Genotype
- Herpesvirus 1, Human
- Kidney
- Mutagenesis
- Restriction Mapping
- Viral Plaque Assay
- Virion