Article
Generation of mutant murine cytomegalovirus strains from overlapping cosmid and plasmid clones.
Journal of virology - 1 Oct 2000
Ehsani M E, Abraha T W, Netherland-Snell C, Mueller N, Taylor M M, Holwerda B
Abstract excerpt
We have developed a cosmid and plasmid system to generate mutant strains of murine cytomegalovirus (MCMV). The system is based on a series of seven overlapping cosmid clones that regenerate MCMV when cotransfected into mouse cells. The unaltered cosmids produce MCMV that is indistinguishable from wild-type MCMV based on restriction enzyme digest patterns of virus DNA and growth rates both in vitro and in vivo....
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