Article
Chimeric molecules created by gene amplification interfere with the analysis of somatic hypermutation of murine immunoglobulin genes.
Gene - 16 May 1994
Ford J E, McHeyzer-Williams M G, Lieber M R
Abstract excerpt
We used the polymerase chain reaction (PCR) to amplify genes encoding murine immunoglobulin (Ig) lambda light-chain variable (V) regions, using DNA isolated from populations of germinal center B-cells, to study somatic hypermutation at this locus. Sequence analysis revealed that 30% of the amplif...
Topics
- Animals
- Artifacts
- B-Lymphocytes
- Base Sequence
- Cloning, Molecular
- DNA Mutational Analysis
- DNA, Recombinant
- Genes, Immunoglobulin
- Immunoglobulin Variable Region
- Immunoglobulin lambda-Chains
- Mice
- Mice, Inbred C57BL
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction