Article
Targeted construction of temperature-sensitive mutations in vaccinia virus by replacing clustered charged residues with alanine.
Proceedings of the National Academy of Sciences of the United States of America - 10 May 1994
Hassett D E, Condit R C
Abstract excerpt
The feasibility of using "clustered charge-to-alanine" mutagenesis (replacement by alanine of two or more charged residues clustered in a five- or six-amino acid sequence) to create temperature-sensitive, conditionally lethal mutations in vaccinia virus was examined by creating nine mutations in...
Topics
- Alanine
- Amino Acid Sequence
- Animals
- Cell Line
- Genes, Dominant
- Genes, Viral
- Isatin
- Kinetics
- Molecular Sequence Data
- Mutagenesis
- Mutation
- Phenotype