Article
Targeted construction of temperature-sensitive mutations in vaccinia virus by replacing clustered charged residues with alanine.
Proceedings of the National Academy of Sciences of the United States of America - 10 May 1994
Hassett D E, Condit R C
Abstract excerpt
The feasibility of using "clustered charge-to-alanine" mutagenesis (replacement by alanine of two or more charged residues clustered in a five- or six-amino acid sequence) to create temperature-sensitive, conditionally lethal mutations in vaccinia virus was examined by creating nine mutations in the vaccinia virus gene G2R. G2R was chosen for this analysis because mutations in this gene confer selectable...
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