Article
A single nucleotide substitution in the transcription start signal of the M2 gene of respiratory syncytial virus vaccine candidate cpts248/404 is the major determinant of the temperature-sensitive and attenuation phenotypes.
Virology - 1 Aug 1998
Whitehead S S, Firestone C Y, Collins P L, Murphy B R
Abstract excerpt
Respiratory syncytial virus (RSV) cpts248/404 is a live-attenuated, temperature-sensitive (ts) vaccine candidate derived from cole-passaged cpRSV by two rounds of chemical mutagenesis and biological selection. Previous sequence analysis showed that these two steps introduced three single nucleoti...
Topics
- Animals
- Antigens, Viral
- Cell Line
- Chickens
- DNA, Complementary
- HN Protein
- Humans
- Mice
- Mice, Inbred BALB C
- Phenotype
- Point Mutation
- Respiratory Syncytial Viruses
- Temperature
- Vaccines, Attenuated
- Viral Envelope Proteins
- Viral Proteins
- Viral Vaccines
- Virus Replication