Clustered charge-to-alanine mutagenesis of human respiratory syncytial virus L polymerase generates temperature-sensitive viruses.

Abstract excerpt

Clustered charge-to-alanine mutagenesis was performed on the large (L) polymerase protein of human respiratory syncytial virus to identify charged residues in the L protein that are important for viral RNA synthesis and to generate temperature-sensitive viruses. Clusters of three, four, and five charged residues throughout the entire L protein were substituted with alanines. A minigenome replicon assay was used...

Topics

• Alanine
• Animals
• Chlorocebus aethiops
• Genome, Viral
• Humans
• Mutagenesis
• Mutagenesis, Site-Directed
• Phenotype
• Rats
• Recombination, Genetic
• Respiratory Syncytial Virus, Human