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Article

A second-site suppressor strategy for chemical genetic analysis of diverse protein kinases.

Nature methods - 1 Jun 2005

Zhang Chao, Kenski Denise M, Paulson Jennifer L, Bonshtien Arale, Sessa Guido, Cross Janet V, Templeton Dennis J, Shokat Kevan M

Abstract excerpt

Chemical genetic analysis of protein kinases involves engineering kinases to be uniquely sensitive to inhibitors and ATP analogs that are not recognized by wild-type kinases. Despite the successful application of this approach to over two dozen kinases, several kinases do not tolerate the necessa...

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Source

Identifiers

PMID: 15908922
DOI: 10.1038/nmeth764

Genes

GRK2

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026. Prepared record publication-15908922-2026-09-02.2. Manifest publication-graph-cohort-2026-09-02.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 2 Sept 2026.

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