Differentially labeled mutant oligonucleotides for analysis of protein-DNA interactions.

Article

Differentially labeled mutant oligonucleotides for analysis of protein-DNA interactions.

BioTechniques - 1 Jan 1994

Freudenreich C H, Kreuzer K N

Abstract excerpt

We have developed a method to produce a set of four duplex oligonucleotides, each with a different labeled base at a given position, from one template-primer combination. The template oligonucleotide is synthesized with a mixture of all four bases at the position of interest, and the primer oligo...

Topics

Base Sequence
DNA
DNA-Binding Proteins
Molecular Sequence Data
Mutation
Oligonucleotides

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