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Article

Phenotypic selection and characterization of mutant alleles of a eukaryotic DNA topoisomerase I.

Genes & development - 1 Apr 1990

Morham S G, Shuman S

Abstract excerpt

We have developed a simple, effective genetic screen for mutant alleles of eukaryotic DNA topoisomerase I that manifest severely depressed or complete loss of enzymatic function. The screen is based on the extreme toxicity of vaccinia topoisomerase expression in the Escherichia coli lysogen strain BL21(DE3) and is notable for its ease in distinguishing nonsense mutations (that result in truncated proteins) from...

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PMID: 2163340
DOI: 10.1101/gad.4.4.515

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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