Phenotypic selection and characterization of mutant alleles of a eukaryotic DNA topoisomerase I.

doi:10.1101/gad.4.4.515

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Article

Phenotypic selection and characterization of mutant alleles of a eukaryotic DNA topoisomerase I.

Genes & development - 1 Apr 1990

Morham S G, Shuman S

Abstract excerpt

We have developed a simple, effective genetic screen for mutant alleles of eukaryotic DNA topoisomerase I that manifest severely depressed or complete loss of enzymatic function. The screen is based on the extreme toxicity of vaccinia topoisomerase expression in the Escherichia coli lysogen strai...

Topics

Alleles
Amino Acid Sequence
Base Sequence
DNA Topoisomerases, Type I
Escherichia coli
Genetic Techniques
Hydroxylamines
Molecular Sequence Data
Mutation
Phenotype
Sequence Homology, Nucleic Acid
Temperature
Vaccinia virus

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