Article
Analysis of phage M13mp2 mutants produced from transfection of phage DNA having N4-aminocytosines at defined sequence positions.
Mutation research - 1 Jul 1992
Matsumoto K, Yashiki T, Bessho T, Negishi K, Hayatsu H
Abstract excerpt
N4-Aminocytidine is mutagenic in various organisms. In the cell, this cytidine analog is metabolized into N4-aminodeoxycytidine 5'-triphosphate, which will then be incorporated into DNA and mutation will result during the replication of the DNA. To prove that the N4-aminocytosine residue in DNA is indeed the site of mutagenesis, we prepared a series of phage M13mp2 DNA samples that bear N4-aminocytosine residues...
Topics
- Bacteriophages
- Base Sequence
- Cytosine
- DNA Polymerase I
- DNA, Single-Stranded
- DNA, Viral
- Escherichia coli
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Transfection