Article
Inhibition of protein synthesis by an efficiently expressed mutation in the yeast 5.8S ribosomal RNA.
Nucleic acids research - 25 Feb 1994
Abou Elela S, Good L, Melekhovets Y F, Nazar R N
Abstract excerpt
Recent studies on the inhibition of protein synthesis by specific anti 5.8S rRNA oligonucleotides strongly suggested that this RNA plays an important role in eukaryotic ribosome function. To evaluate this possibility further, a ribosomal DNA transcription unit from Schizosaccharomyces pombe was cloned into yeast shuttle vectors with copy numbers ranging from 2 to approximately 90 per cell; to allow direct...
Topics
- Base Sequence
- Fungal Proteins
- Kinetics
- Molecular Sequence Data
- Mutation
- Protein Biosynthesis
- RNA, Fungal
- RNA, Ribosomal, 5.8S
- Schizosaccharomyces